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Micromachined adjustable hydrogel stamper for soft printing of biomolecules with adjustable-feature dimensions.

Wan Salim, Wan Wardatul Amani and Ding, Zenwen and Ziaie, Babak (2009) Micromachined adjustable hydrogel stamper for soft printing of biomolecules with adjustable-feature dimensions. Analytical Chemistry, 81 (11). pp. 4551-4554. ISSN 0003-2700

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Abstract

We report on the development of a hydrogel stamper with built-in reservoirs for soft-printing of biomolecules with adjustable feature dimensions. The stamper consists of potassium hydroxide (KOH)-etched silicon cavities onto which biomolecule-soaked low cross-linked density hydrogel with large pores and low mechanical strength is loaded. Application of weight to the top surface allows for a controllable protrusion of hydrogel from the opposite nozzles. Such protrusion combined with a suitable spacer between the stamper and a substrate provides a means for printing features with dimensions depending on the applied weight. Utilizing the above method, we successfully stamped bovine serum albumin conjugated with fluorescein isothiocyanate (BSA-FITC) model proteins on hydrophilic silicon substrates with a feature dimension ratio of 20:1 using a single stamper.

Item Type: Article (Journal)
Additional Information: 7116/44326
Uncontrolled Keywords: Bovine serum albumins; Feature dimensions; Fluorescein isothiocyanate; Large poresMechanical strength; Micromachined; Model proteinsSilicon cavitySilicon substratesTop surface
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Wan Wardatul Amani Wan Salim
Date Deposited: 02 Oct 2017 15:56
Last Modified: 02 Oct 2017 15:56
URI: http://irep.iium.edu.my/id/eprint/44326

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