Development of a module for cell culture based on a decellularized vascular tissue scaffold

Ataru , MURAHARA and Akimitsu , WAKIDA and Yu , MIYAKE and Shoichiro , TATEISHI and Takahiro , HIRAYAMA and Noor Azmi, Azran Azhim and Kazuaki , MURAMATSU and Masato , TANAKA and Osamu , ENDO and Kozo , MIYAZAKI and Hirotaka , MATSUO and Nariyoshi , SHINOMIYA and Yuji , MORIMOTO (2011) Development of a module for cell culture based on a decellularized vascular tissue scaffold. Transactions of Japanese Society for Medical and Biological Engineering , 49 (3). pp. 508-515. ISSN 1881-4379

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Abstract

Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx.

Item Type:	Article (other)
Additional Information:	7380/44074
Uncontrolled Keywords:	detergent, porcine, vascular tissue, calcium
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	11 Aug 2015 08:42
Last Modified:	11 Aug 2015 08:42
URI:	http://irep.iium.edu.my/id/eprint/44074

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