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The use of sonication treatment to completely decellularize blood arteries: a pilot study

Noor Azmi, Azran Azhim and Yamagami, K. and Muramatsu, K. and Morimoto, Y. and Tanaka, Masato (2011) The use of sonication treatment to completely decellularize blood arteries: a pilot study. In: 33rd Annual International Conference of the IEEE EMBS, 30 Aug-3 Sept. 2011, Boston, Massachusetts.

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We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, sonication power and SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) staining and scanning electron microscopic (SEM) photographs. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. Decellularization efficiency was tended to increase significantly when DO value decreasing after 6 hours of treatment. In conclusion, we conclude that sonication treatment can be used to prepare the complete decellularized scaffolds in short treatment time.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 7380/44031 (ISBN: 978-1-4244-4122-8, DOI: 10.1109/IEMBS.2011.6090685)
Uncontrolled Keywords: biological effects of acoustic radiation biomedical ultrasonics blood vessels cavitation scanning electron microscopy tissue engineering
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Depositing User: Ir Dr Azran Azhim Noor Azmi
Date Deposited: 06 Aug 2015 14:23
Last Modified: 06 Aug 2015 14:23
URI: http://irep.iium.edu.my/id/eprint/44031

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