Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae

Mohamed Shakrin, Nik Noorul Shakira and Balasubramaniam, Shandra Devi and Ahmad Yusof, Hanani and Mastuki, Mohd Fahmi and Masri, Siti Norbaya and Mohd Taib, Niazlin and Amin Nordin, Syafinaz and Jamal, Farida and Clarke, Stuart Charles and Mohd Desa, Mohd Nasir (2013) Evaluation of PCR-based approach for serotype determination of streptococcus pneumoniae. Tropical Biomedicine, 30 (2). pp. 338-344. ISSN 0127-5720, OCLC: 12391449

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Abstract

Determination of Streptococcus pneumoniae serotypes is essential for epidemiological surveillance. Therefore accurate, reliable and cost effective serotyping method is crucial. In this study, we determined the serotypes of 41 pneumococcal isolates recovered from human anterior nares by multiplex Polymerase Chain Reaction (PCR) utilizing published primers. The data was then compared with conventional serology using latex agglutination (LA) and the Quellung reaction. Based on the PCR-approach, 8 different serogroups/serotypes were detected with one isolate classified as non-typeable (cpsAnegative). In reference to the serology-based data, the results were in agreement except for one isolate. For the latter isolate, the LA and Quellung tests failed to show a reaction but the PCR-approach and sequencing identified the isolate as serogroup 15B/C. Based on this experimental setting, we found that the PCR-approach for pneumococcal serotypes determination is reliable to serve as the alternative for determining the pneumococcal serotyping.

Item Type:	Article (Journal)
Additional Information:	7232/43517
Uncontrolled Keywords:	SEQUENTIAL MULTIPLEX PCR; DETERMINING CAPSULAR SEROTYPES; VIRULENCE FACTORS; DISEASE; CHILDREN; COLONIZATION; PREVALENCE
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	24 Jun 2015 14:53
Last Modified:	24 Jun 2015 14:53
URI:	http://irep.iium.edu.my/id/eprint/43517

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