Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts

Jalal, Tara Kamal and Ahmed, Idris Adewale and Mikail, Maryam Abimbola and Momand, Lailuma and Draman, Samsul and Md Isa, Muhammad Lokman and Abdull Rasad, Mohammad Syaiful Bahari and Omar, Muhammad Nor and Ibrahim, Muhammad and Abdul Wahab, Ridhwan (2015) Evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. Applied Biochemistry and Biotechnology, 175 (7). pp. 3231-3243. ISSN 0273-2289

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Abstract

Artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol. The antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test. IC50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. In the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (Trolox) and 88.34 ± 1.31 % for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteu procedure; methanol pulp part demonstrated the highest phenol content value of 781 ± 52.97 mg GAE/g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay, the highest value of 6213.33 ± 142.22 mg QE/g was indicated by pulp part of the fruit methanol extract. The antimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Salmonella typhimurium, Escherichia coli, Klebsiella pneumonia and Candida albicans. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration (MIC) and MBC/minimal fungicidal concentration (MFC) for the extracts were also determined using the microdilution method ranging from 4000 to 63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have potential bioactivities due to high content of phenolic compounds.

Item Type:	Article (Journal)
Additional Information:	5136/43516 Corresponding author: Dr.Ridhwan Abdul Wahab
Uncontrolled Keywords:	Artocarpus altilis, Antioxidants, DPPH, Antimicrobial, MIC and MBC/MFC
Subjects:	R Medicine > RL Dermatology R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Medicine > Department of Family Medicine (Effective: 1st January 2011) Kulliyyah of Nursing Kulliyyah of Science > Department of Biotechnology Kulliyyah of Allied Health Sciences Kulliyyah of Medicine Kulliyyah of Science
Depositing User:	Dr Ridhwan Abdul Wahab
Date Deposited:	24 Jun 2015 09:28
Last Modified:	08 Jun 2022 16:05
URI:	http://irep.iium.edu.my/id/eprint/43516

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