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Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells

Haque, Quazi Manjurul and Mohammad , Najmiyatul Fadilah and Helaluddin , Abul Bashar Mohammed and Saeed , Mohammad (2010) Cytotoxicity of Vibrio cholerae of Madin Darby bovine kidney cells. Pakistan Journal of Pharmaceutical Sciences, 23 (4). pp. 393-397. ISSN 11011-601X

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Abstract

The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5- dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to detect the effect of the filtrates on the proliferation and viability of cultured cell populations. The filtrates were prepared from serial ten-fold dilutions of inoculated AKI and APW broth media with and without the addition of polymyxin B. The APW culture filtrates of both V. cholerae O1 and O139 with and without added polymyxin B showed greater toxicity to MDBK cells as compared to AKI filtrates. The cytotoxicity of AKI-grown V. cholerae O139 to MDBK cells was greater than that of V. cholerae O1 grown in the same medium. The cytotoxicity of APW filtrates on Vero cells was low and only noted when polymyxin was added to the medium. Keywords: Vibrio cholerae, MDBK cells, Vero cells, Cytotoxicity and MTT test.

Item Type: Article (Journal)
Additional Information: 4478/4307
Uncontrolled Keywords: Vibrio cholerae, MDBK cells, Vero cells, cytotoxicity and MTT test
Subjects: Q Science > QR Microbiology > QR355 Virology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 31 Oct 2011 09:37
Last Modified: 29 Oct 2013 15:57
URI: http://irep.iium.edu.my/id/eprint/4307

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