Bioguided isolation of cytotoxic triterpenoids from the stem of luvunga scandens

Al-Zikri, Putri Nurhidayah and Bakhtiar, M. Taher and Darnis, Deny Susanti and Rezali, Mohd Fazlin and Read, Roger W and Sohrab, Md. Hossain and Hasan, Choudhury Mahmood and Ahmad, Farediah (2015) Bioguided isolation of cytotoxic triterpenoids from the stem of luvunga scandens. In: International Conference on Natural Products 2015, 24-25 March 2015, Double Tree by Hilton, Johor Bahru.

	PDF - Published Version Restricted to Repository staff only Download (14MB) | Request a copy
	PDF - Supplemental Material Restricted to Repository staff only Download (2MB) | Request a copy

Abstract

Luvunga scandens species belongs to the family of Rutaceae. This plant usually inhabit tropical and moist environment and known as ‘Mengkurat Jakun’ among locals. A bioactivity-guided isolation and chemical investigation of the stem of Luvunga scandens resulted in the isolation and characterization of triterpenoids, 3-oxotirucalla-7,24-dien-21-oic-acid (1), and flindissol (2). The structures of the compound were determined on the basis of spectroscopic analyses including 2D NMR. The inhibitory effects of 1–2 on the proliferation on human breast adenocarcinoma cell line (MCF-7) have been evaluated by MTT assay and their effects on cell cycle and apoptosis investigated by flow cytometry with the morphological observation under Scanning Electron Microscope (SEM). Compounds 1 and 2 showed potent cytotoxicity against MCF-7 cell line with IC50 values of 27.5 µM and 13.8 µM respectively. Flow cytometry investigations for 1–2 demonstrated that both compounds exert their anti-proliferative effect on MCF-7 cells through inhibiting cell cycle and inducing apoptosis. This result suggested that both compounds might be useful for anticancer agents.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	5209/42493
Uncontrolled Keywords:	cytotoxic triterpenoids
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Muhammad Taher
Date Deposited:	06 Aug 2015 09:59
Last Modified:	23 May 2016 15:01
URI:	http://irep.iium.edu.my/id/eprint/42493

Actions (login required)

View Item