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In vitro wound healing activity of sapium indicum willd leaf extracts

Abdul Aziz, Huwaida and Bakhtiar, M. Taher and Darnis, Deny Susanti and Zakaria, Zainul Amiruddin (2015) In vitro wound healing activity of sapium indicum willd leaf extracts. African Journal of Traditional, Complementary and Alternative Medicines, 12 (2). pp. 126-132. ISSN 0189-6016

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Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community. Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model. Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity (MTT assay) and scratch assay. Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI exhibited IC50 = 16.3 and 70.9 μg/mL, respectively. In the scratch assay, EESI caused significant (P< 0.05) concentration-dependent migration on 3T3 L1 cells whereas AESI exerted concentration-independent effect. Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These findings would support potential use of S. indicum as wound healing plant.

Item Type: Article (Journal)
Additional Information: 5209/42490
Uncontrolled Keywords: Sapium indicum, cell migration, in vitro wound healing
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 23 Apr 2015 10:19
Last Modified: 16 May 2016 10:30
URI: http://irep.iium.edu.my/id/eprint/42490

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