Host-specific microbial communities in three sympatric North Sea sponges

Naim, Mohd Azrul and Morillo, Jose A. and Sørensen, Søren J. and Waleed, Abu Al-Soud and Smidt, Hauke and Sipkema, Detmer (2014) Host-specific microbial communities in three sympatric North Sea sponges. FEMS Microbiology Ecology, 90 (2). pp. 390-403. ISSN 0168-6496

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Abstract

The establishment of next-generation technology sequencing has deepened our knowledge of marine sponge-associated microbiota with the identification of at least 32 phyla of Bacteria and Archaea from a large number of sponge species. In this study, we assessed the diversity of the microbial communities hosted by three sympatric sponges living in a semi-enclosed North Sea environment using pyrosequencing of bacterial and archaeal 16S ribosomal RNA gene fragments. The three sponges harbor species-specific communities each dominated by a different class of Proteobacteria. An -proteobacterial Rhodobacter-like phylotype was confirmed as the predominant symbiont of Halichondria panicea. The microbial communities of Haliclona xena and H.oculata are described for the first time in this study and are dominated by Gammaproteobacteria and Betaproteobacteria, respectively. Several common phylotypes belonging to Chlamydiae, TM6, Actinobacteria, and Betaproteobacteria were detected in all sponge samples. A number of phylotypes of the phylum Chlamydiae were present at an unprecedentedly high relative abundance of up to 14.4 +/- 1.4% of the total reads, which suggests an important ecological role in North Sea sponges. These Chlamydiae-affiliated operational taxonomic units may represent novel lineages at least at the genus level as they are only 86-92% similar to known sequences.

Item Type:	Article (Journal)
Additional Information:	7319/42287
Uncontrolled Keywords:	sponge microbiota, pyrosequencing, host-specific, microbial diversity, Chlamydiae, Proteobacteria
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User:	Dr Mohd Azrul Naim Mohamad
Date Deposited:	06 Mar 2015 15:32
Last Modified:	11 Jun 2018 10:59
URI:	http://irep.iium.edu.my/id/eprint/42287

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