Preliminary study on biohydrogen production by bacteria from sago waste

Mohamad Puad, Noor Illi and Sulaiman, Sarina and Azmi, Azlin Suhaida and Shamsudin, Yusra and Mel, Maizirwan (2014) Preliminary study on biohydrogen production by bacteria from sago waste. In: 27th Symposium of Malaysian Chemical Engineers (SOMChE 2014) in conjunction with 21st Regional Symposium on Chemical Engineering (RSCE 2014), 28th-30th Oct. 2014, Taylor's University Lakeside Campus, Subang Jaya, Selangor. (Unpublished)

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Abstract

Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia coli (E. coli) using dark fermentation by utilizing sago waste as the substrate. Acid pre-treatment process for sago waste was executed and the content of reducing sugar was analysed using Dinitrosalicylic acid (DNS) method. The glucose content after pre-treatment process was 2.150 g/L when treated at the temperature of 90C for 40 minutes. Dark fermentation process was performed for 14 hours and three factors which were inoculum size (10-30%), pH (6-8) and temperature (28-40C) were investigated. The design of experiment was done using central composite design (Design Expert v6.0.8). Maximum yield of 2.22 mol H2/ mol glucose was obtained from Run 8 at processing parameters of pH 6, temperature of 40C and 30% inoculum size. The findings of this study can be applied in production of biohydrogen at a wider range of parameters (pH, temperature, inoculum size) for further studies.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	5462/42264
Uncontrolled Keywords:	Biohydrogen, Escherichia coli, Sago waste, Fermentation.
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	23 Mar 2015 09:26
Last Modified:	18 Jun 2018 21:39
URI:	http://irep.iium.edu.my/id/eprint/42264

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