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Mechanism in the clot formation of horseshoe crab blood during bacterial endotoxin invasion

John, Akbar and Khan Chowdhury, Ahmed Jalal and Yunus, Kamaruzzaman and Kasim, Zaleha (2010) Mechanism in the clot formation of horseshoe crab blood during bacterial endotoxin invasion. Journal of Applied Sciences, 10 (17). pp. 1930-1936. ISSN 1812-5662 (O), 1812-5654 (P)

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Endotoxins from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LAL tests in general were 3 to 300 times more sensitive than the United States Pharmacopeial (USP) rabbit pyrogen test method. It should be noted that major differences among the LAL preparations lie in the area of sensitivity. Differences, up to 100-fold, exist in the sensitivity of the various LAL preparations to the same endotoxin. It is also considerate that the Information on the molecular level interaction between horseshoe crab blood and endotoxin would provide detailed information about the clotting process. Based on the above perspective review of literature was established to elucidate the compounds involved in the clot formation in horseshoe crab blood while encountering with bacterial endotoxin. Since LAL preparation is important for its application in clinical, pharmaceutical, public health and environmental areas, this paper will also discuss some molecular level details in Lysated Amebocyte interaction in clot formation

Item Type: Article (Journal)
Additional Information: 3575/4215
Uncontrolled Keywords: Endotoxin, LAL test, horseshoe crab, amebocyteltsate, pyrogen test
Subjects: Q Science > QL Zoology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Science > Institute of Oceanography and Maritime Studies
Depositing User: Professor Ahmed Jalal Khan Chowdhury
Date Deposited: 17 Oct 2011 12:40
Last Modified: 07 Dec 2017 12:55
URI: http://irep.iium.edu.my/id/eprint/4215

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