High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector

Helaluddin , Abul Bashar Mohammed and Mohamad , Huda Jamilah and Alaama, Mohammed and Amiruddin , Noor Syafawati (2014) High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector. In: International Conference on Pharmaceutical, Medical & Environmental Health Sciences (IPharME, 2014), 17-18 October, 2014, Park Royal, Penang.

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Abstract

Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. A simple and sensitive high-performance liquid chromatography-UV detection method was developed for the detection of mefenamic acid. Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i.d 3.5µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored under 280 nm using variable-wavelength detector. The recovery was found to be 83% for MA. The method was validated for following the CDER (Centre for Drug Evaluation and Research) guideline. Calibration plot were linear within the range from 250 to 5000ng ml-1 with the correlation coefficient (r2) of ≥ 0.99. The result from analysis of quality control of mefenamic acid which were termed as low, medium and high were analysed to get the precision and accuracy. The accuracy for intra-day for low, medium and high were 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient of variance (CV) for intra-day precision for low, medium and high were found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keyword Mefenamic acid, HPLC, plasma, protein precipitation technique.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4478/41453
Uncontrolled Keywords:	Mefenamic acid, HPLC, plasma, protein precipitation technique
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. A. B. M. Helal Uddin
Date Deposited:	23 Apr 2015 12:34
Last Modified:	23 Apr 2015 12:34
URI:	http://irep.iium.edu.my/id/eprint/41453

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