Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering

Ahmad Radzi, Muhammad Aa'zamuddin and Jamaludin, Fatin Nabila and Abdul Rahman, Rozlin and Md Nazir, Noorhidayah and Mohamad Sukri, Norhamiza and Mohammad, Mohd Yusof and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Hashi, Abdurezak Abdulahi and Sha'ban, Munirah (2014) Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering. Regenerative Research, 3 (2). pp. 118-119. ISSN 2232-0822 (Online)

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Abstract

Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essential Medium (α-MEM) and, (2) the mixture of Ham F12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (FD). Growth kinetics assessment used in this study includes cell viability, total cell yield, growth rate, population doubling time and total number of cell doubling. FD was found to be an appropriate culture medium to promote better BMMSC growth. BMMSC from iliac crest region showed better proliferation potential than the femur region. Hence, it can be inferred from this study that BMMSC harvested from the iliac crest site and cultured in FD medium is better than femur samples in terms of growth profile.

Item Type:	Article (Journal)
Additional Information:	6006/41425
Uncontrolled Keywords:	Bone marrow, mesenchymal stem cells,growth kinetics, -MEM, F12:DMEM
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Munirah Sha'ban
Date Deposited:	12 Feb 2015 16:09
Last Modified:	12 Feb 2015 16:09
URI:	http://irep.iium.edu.my/id/eprint/41425

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