Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds

Md Nazir, Noorhidayah and T. M., Tengku Nuramirah and Razak, Syahidah and Mohamad, Mohd Yusof and Abdul Rahman, Rozlin and Mohamad Syukri, Norhamiza and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2014) Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds. Regenerative Research, 3 (2). pp. 46-47. ISSN 2232-0822 (Online)

Preview

PDF (Journal Containing Extended Abstract) - Published Version Download (284kB) | Preview

Abstract

Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffolds and biologically active signalling factors for in vitro and in vivo IVD generation. This present study aimed to evaluate the cartilaginous properties of nucleus pulposus (NP) cells seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin scaffolds by measuring sulphated glycosaminoglycan (sGAG) content and collagen type II (COL-II) gene expression. The analyses were performed at week 1, 2, and 3 in vitro. The sGAG content was higher in the PLGA/Fibrin than in the PLGA only group. The expression of COL-II was only detected at week-2 culture in PLGA/Fibrin group. This study indicates that PLGA-based scaffolds may have the potential for intervertebral discs tissue engineering.

Item Type:	Article (Journal)
Additional Information:	6006/41420
Uncontrolled Keywords:	Tissue engineering, Intervertebral Disc, Nucleus pulposus
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Munirah Sha'ban
Date Deposited:	12 Feb 2015 17:07
Last Modified:	12 Feb 2015 17:07
URI:	http://irep.iium.edu.my/id/eprint/41420

Actions (login required)

View Item