Methylation specific-high resolution melting analysis of DISC1

Abd. Rahim, Nour El Huda and Ku Zaifah, Norsidah and A.Talib, Norlelawati (2014) Methylation specific-high resolution melting analysis of DISC1. In: International Medical Genetics Conference (IMGC 2014), 3-5 December 2014, Putra World Trade Centre. (Unpublished)

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Abstract

Introduction: The evidence of Disrupted in Schizophrenia 1 (DISC1) gene’s role in schizophrenia came through functional and animal studies. However the exact genetic basis that contribute to the role is still not established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. Objectives: We aim to explore the epigenetic dysregulation as the basis of DISC1 gene contribution to schizophrenia. To achieve this aim, method for quantitation of DISC1’s DNA methylation has to be established. Methods: The high resolution melting (HRM) analysis method was adopted for DNA methylation assay. Primers were designed based on Wojdacz’s protocol with some modifications. The human methylated and unmethylated control DNA samples were titrated into serial methylation percentage of 0%, 25%, 50%, 75% and 100% and were bisulfite treated. The methylation specific-high resolution melting (MS-HRM) assay for DISC1 was optimized based on its response to various percentage of DNA methylation. Results: The optimized protocol clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. Conclusion: This optimized HRM protocol is to be used for the actual MS-HRM analysis of the DISC1 in schizophrenia.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	6300/40632
Uncontrolled Keywords:	Epigenetic, HRM, DNA methylation, Schizophrenia
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Dr Nour El Huda Abd Rahim
Date Deposited:	10 Mar 2015 10:30
Last Modified:	23 May 2018 14:17
URI:	http://irep.iium.edu.my/id/eprint/40632

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