High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector

Helal Uddin, A.B.M. and Mohamad, Huda Jamilah and Alaama, Mohammed and Amiruddin, Noor Syafawati (2014) High performance liquid chromatographic determination of mefenamic acid in human plasma using UV vis detector. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (11). pp. 167-170. ISSN 0975-1491

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Abstract

Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. Methods: Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 Results: The recovery was found 83% for MA. The method was validated according to the Centre for Drug Evaluation and Research (CDER) guidelines. Calibration plot was linear within the range from 250 to 5000ng ml(150 mm x 4.6 mm, i. d 3.5 μm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored at 280 nm using variable-wavelength detector. -1 with the coefficient of determination (r2 Conclusion: The results were in compliance with CDER guideline. ) of ≥ 0.99. The quality control samples of mefenamic acid which was termed as low (L), medium (M) and high (H) were analysed to get the precision and accuracy. The accuracy for intra-day for L, M and H was 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient variance (CV) for intra-day precision for L, M and H was found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keywords: HPLC, Mefenamic Acid, Human plasma, UV detector, Protein precipitation.

Item Type:	Article (Journal)
Additional Information:	4478/40411
Uncontrolled Keywords:	HPLC, Mefenamic Acid, Human plasma, UV detector, Protein precipitation
Subjects:	Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. A. B. M. Helal Uddin
Date Deposited:	30 Dec 2014 12:58
Last Modified:	07 Nov 2022 11:27
URI:	http://irep.iium.edu.my/id/eprint/40411

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