Bioassay guided isolation of an antidermatophytic active constituents from the stembarks of entada spiralis ridl

Harun, Aiza and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian and Che Mohd Ramli, Neni Kartini (2014) Bioassay guided isolation of an antidermatophytic active constituents from the stembarks of entada spiralis ridl. In: Regional Conference On Science, Technology & Social Sciences , 23rd - 25th November 2014, Cameron Highlands, Pahang. (Unpublished)

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Abstract

Entada spiralis ridl (lenguminoceae) is a liana or woody climber that grows in the wild in Malaysia and is known locally as "Beluru" or "Sintok". The isolation and characterization of the chemical constituent from an active fraction of E. spiralis stem bark has been carried out. Our previous study revealed that methanol extract of E. spiralis stem bark exhibited promising antifungal activity against three dermatophyte strains, namely Trichophyton mentagrophytes ATCC 9533, Trichophyton tonsurans ATCC 28942 and Microsporum Gypseum ATCC 24102 that cause skin infection. This study was performed to elucidate the structure of active constituent known as ester saponin from the active fraction of E. spiralis stem bark. The factions were prepared using fractionation process and repeated antifungel test was conducted to identify the most active fraction. The structure elucidation of this compound was based on spectroscopis data (1H, 13C NMR, HMQC, HMBC and DEPT135) and comparison with literature. on the basis of spectroscopis analysis, the compound was identified as 28-α,L-rhamnopyranosyl-18,21,22-trihydroxy-12-en-29-(2-acetylamino-β-Dglucopyranosyl)triterpene ester. The current study provides important baseline information for the use of E. spiralis for the treatment of skin infection caused by the microorganism investigated in this study.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5652/40051
Uncontrolled Keywords:	Antifungal activity, Dermatophytes, Entada spiralis, Lenguminoceae, Terpenoid
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Siti Zaiton Mat So'ad
Date Deposited:	23 Dec 2014 12:30
Last Modified:	06 Jan 2015 15:34
URI:	http://irep.iium.edu.my/id/eprint/40051

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