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Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres

Rahman, Md. Mokhlesur and Mohamed, Awang and Haliza, Katas and Ansary , Rezaul H. (2014) Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres. In: Malaysia International Biological Symposium 2014 (i-SIMBIOMAS 2014), 28th - 29th October 2014, Putrajaya.

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In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Additional Information: 5269/39998 -- MALAYSIA INTERNATIONAL BIOLOGICAL SYMPOSIUM 2014 “Sustainable Bioresources for Bioeconomy” PROCEEDINGS
Uncontrolled Keywords: bovine serum albumin
Subjects: Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr Mohd Mokhlesur Rahman
Date Deposited: 17 Dec 2014 15:38
Last Modified: 11 Jun 2018 11:48
URI: http://irep.iium.edu.my/id/eprint/39998

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