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Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy

Doolaanea, Abd Almonem and Harun Ismail, Ahmad Fahmi and Mohamed, Farahidah (2014) Quantification of nigella sativa oil (NSO) from biodegradable PLGA nanoparticles using FTIR spectroscopy. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (10). pp. 228-232. ISSN 0975-1491

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Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent. Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations. Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage.

Item Type: Article (Journal)
Additional Information: 4341/39644
Uncontrolled Keywords: Nigella sativa, FTIR, Loading efficiency, PLGA, Chitosan, Nanoparticle
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr. Abd Almonem Doolaanea
Date Deposited: 15 Dec 2014 09:57
Last Modified: 11 Mar 2015 16:36
URI: http://irep.iium.edu.my/id/eprint/39644

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