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Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application

Mohd Shafri, Mohd Affendi and Mohamed, Farahidah and Yusof, Fatin Athirah and Yaacob, Khairul Ikhwan and Mohd Yusof, Nazri (2014) Formulation and stability testing of Gentamicin-N. sativa fusion emulsions for osteo-healing application. International Journal of Pharmacy and Pharmaceutical Sciences, 6 (11). pp. 171-176. ISSN 0975-1491

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Abstract

An alternative osteo-healing formulation with osteo-healing properties was formulated by combining gentamicin and Nigella sativa (N. sativa) oil in an emulsion to reduce gentamicin toxicity effect over prolonged use in osteo-infection treatment. This work aims to test the aqueous solubility and physicochemical properties of the emulsion. Four emulsions (emulsion A, B, C and D) had been formulated, with final concentration of gentamicin was made constant at 0.1% (w/v) whereas N. sativa oil concentration was varied between 32.5% (v/v) to 46.4% (v/v) in all formulations. Then, stability studies of all emulsion were performed by centrifugation at (5000rpm, 5 minutes), at different storage conditions (8oC, 25oC and 50oC), organoleptic characteristics, freeze-thawcycle, pH determination, particle size measurement, zeta-potential analysis, and pH titration analysis. Results showed no phase separation after centrifugation for freshly prepared emulsions. Storage at 8oC, all emulsions also showed no phase separation at all-time points. At 25oC storage condition, three formulations were stable at day 7 but phase separation was formed in all emulsions by day 14showed good stability at day 7 and all emulsions formed phase separation at day 14. No emulsions were stable in storage temperature of 50oC. The particle size of the emulsions increased with an increment ofN. sativa oil concentration. Zeta-potential analysis showed a range of -32.2 ± 0.15 mV to -48.0 ± 0.45 mV. When pH titration analysis was performed, the zeta potential indicated that the emulsion stability was affected by acidic conditions. We concluded that the use of gentamicin-N. Sativaemulsions must take into account the storage condition with preference of low temperature and fresh preparation at higher alkalinity and the lowest possibility of N. sativa oil.

Item Type: Article (Journal)
Additional Information: 6845/39609
Uncontrolled Keywords: Gentamicin, Nigellasativa, Emulsion, Stability, Osteo-healing.
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Mohd Affendi Mohd Shafri
Date Deposited: 10 Dec 2014 15:46
Last Modified: 07 Sep 2017 16:25
URI: http://irep.iium.edu.my/id/eprint/39609

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