Perumal, Vikneswari and Khatib, Alfi and Uzir, Bisha Fathamah and Abas, Faridah and Bajrai, Aishah and Ahmed, Qamar Uddin and Nik Yusoff, Nik Mohd. Idris
(2014)
Multivariate calibration of fourier transform infrared spectra in predicting anti-diabetic activity of momordica charantia fruit.
In: International Health Conference IIUM 2014, 3rd - 4th December 2014, Kuantan, Pahang.
Abstract
Momordica charantia or bitter melon is widely used as traditional medicine for diabetic
patient. This study developed a statistical model in predicting the alpha-glucosidase
inhibitory activity of M. charantia fruit based on Fourier Transform Infrared Spectroscopy
(FTIR). A total of six different ethanol extracts (0, 20, 40, 60, 80, and 100%) of this plant
were prepared. The chemical profiles of the extracts was conducted using FTIR spectroscopy
between 4000 and 600/cm at a resolution of 4/cm. Multivariate Data Analysis (MVDA) was
developed by correlating the bioactivity and the third derivative FTIR spectra of each
individual extract using Orthogonal Partial Least Square (OPLS). The result revealed that the
regression coefficient (R2) between actual and predicted α-glucosidase inhibitory activity
was high (>0.80), and the standard error of prediction (SEP) and standard error of estimation
(SEE) were acceptable. In conclusion, FTIR spectroscopy is a cheap and high-throughput
technique that can be used as to predict the α-glucosidase inhibition activity of M. charantia
fruit .
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