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The growth inhibitory effect of Henna (L.inermis) Aqueous extract in human lung cancer cells, A549

Abdul Ghani, Radiah and Nik Abdul Malek, Nik Nurasyikin (2014) The growth inhibitory effect of Henna (L.inermis) Aqueous extract in human lung cancer cells, A549. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan, Pahang. (Unpublished)

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Abstract

Chemotherapy is the common treatment used to handle cancer cases. Since the drugs used in chemotherapy act on human body by damaging high proliferating cells, normal cell for instance bone marrow, hair follicles and gastrointestinal mucosa which undergoes high rates of cell division are most susceptible. It also produces common side effects like neutropenia, anorexia, diarrhea and many others. In recent study, Lawsoniainermisor henna can become one of the potential natural therapies to treat common cancer cells (Pradhan et al., 2012). Many studies had explored the properties of henna for its scientific components by extracting certain compound but did not associate the usage of all components of henna in one study. This study was carried out with the aims to observe the pattern of A549 cells growth before and after being treated with henna and to determine the inhibitory effects of boiled henna liquid in human lung adenocarcinoma epithelial cells (A549). To compare the A549 cell growth before and after the addition of henna, cell number and protein content of the culture were analyzed. Boiled liquid of henna was used so that all of the components would be extracted in the liquid. MTT assay, trypan blue exclusion method and Lowry assay were employed to identify the inhibitory effects of boiled henna, to obtain the standard growth curve and to assess the protein content respectively. The result revealed that henna has the ability to inhibit the growth of A549 cells by showing an IC50 value of 5% after 24 hours exposure and 2% after 48 and 72 hours exposure. The growth curve obtained showed a significant difference in inhibition of A549 cell growth after been exposed to henna compared to untreated A549 (p<0.05). The protein content had a moderate correlation with cell number after being treated with boiled henna (r2= 0.4966). In conclusion, the findings revealed that henna has cytotoxic effects against A549 cell lines. This basic study will help in providing scientific justification for the use of this prophetic medicine in treating cancer. Keywords:Lawsoniainermis, henna, inhibitory effects, MTT assay,A549

Item Type: Conference or Workshop Item (Poster)
Additional Information: 4458/39526
Subjects: R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Radiah Abdul Ghani
Date Deposited: 09 Dec 2014 09:26
Last Modified: 24 May 2018 09:00
URI: http://irep.iium.edu.my/id/eprint/39526

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