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In vitro degradation study of novel HEC/PVA/collagen nanofibrous scaffold for skin tissue engineering applications

Zulkifli, Farah Hanani and Jahir Hussain, Fathima Shahitha and Abdull Rasad, Mohammad Syaiful Bahari and Mohd Yusoff, Mashitah (2014) In vitro degradation study of novel HEC/PVA/collagen nanofibrous scaffold for skin tissue engineering applications. Polymer Degradation and Stability, 110. pp. 473-481. ISSN 0141-3910

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Abstract

The aim of this study was focused on the degradation behavior of electrospun (hydroxyethyl cellulose/poly(vinyl) alcohol) HEC/PVA and HEC/PVA/collagen nanofibrous scaffolds, as a potential substrates for skin tissue engineering in two biologically related media: phosphate buffered solution (PBS) and Dulbecco's modified Eagle's medium (DMEM) for 12 weeks incubation period. The scaffolds were characterized at different degradation times by a series of analysis including pH changes of solutions, weight loss, swelling ratio, SEM, ATR-FTIR, DSC, TGA and mechanical properties. The results indicated that HEC/PVA/collagen scaffolds were exhibited slower degradation rate in both medium as compared to HEC/PVA blend nanofibers. All fibers displayed uneven and rough surfaces towards the final week of incubation in both PBS and DMEM solution. As degradation time increased, there were little changes in the chemical structure as determined by FTIR spectra while thermal studies revealed that the melting temperatures and crystallinity of scaffolds were slightly shifted to a lower value. Both HEC/PVA and HEC/PVA/collagen fibers showed significant decrease in Young's modulus and tensile stress over 12 weeks degradation. These results show that these nanofibrous scaffold demonstrate degradation behavior that meets the requirement as potential degradable biomaterials for dermal replacement.

Item Type: Article (Journal)
Additional Information: 4701/39192
Uncontrolled Keywords: In vitro degradation; Hydroxyethyl cellulose; Collagen; Nanofibers scaffold; Tissue engineering
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Mohammad Syaiful Bahari Abdull Rasad
Date Deposited: 18 Nov 2014 14:42
Last Modified: 28 Oct 2020 11:33
URI: http://irep.iium.edu.my/id/eprint/39192

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