Biotransformation of ω fatty acids using pseudomonas aeruginosa

Omar, Muhammad Nor (2012) Biotransformation of ω fatty acids using pseudomonas aeruginosa. In: International Biotechnology Symposium and Exhibition (IBS 2012), 16-21 September 2012, Daegu, Korea. (Unpublished)

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Abstract

Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the freeze-dried catfish flesh using a modified Folch method with the chloroform and methanol system. The crude oil was added to the bacterial culture and incubated at 28oC for 4 days. After incubation, the biotransformed product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to hydroxyl fatty acids. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5626/39021
Uncontrolled Keywords:	Biotransformation, Pseudomonas aeruginosa, ω fatty acids
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	06 Nov 2014 16:20
Last Modified:	06 Nov 2014 16:20
URI:	http://irep.iium.edu.my/id/eprint/39021

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