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In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts

Abdull Rasad, Mohammad Syaiful Bahari and Halim, Ahmad Sukari and Hashim, Kamaruddin and Abdul Rashid , Ahmad Hazri and Yusof, Norimah and Shamsuddin, Shaharum (2010) In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydrate Polymers, 79. pp. 1094-1100. ISSN 0144-8617 (O)

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Abstract

The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level.

Item Type: Article (Journal)
Additional Information: 4701/38238
Uncontrolled Keywords: Cytocompatibility, Chitosan derivatives, Sheet, Paste Primary human fibroblasts, Hypertrophic scars, Matrix metalloproteinase-13
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Mohammad Syaiful Bahari Abdull Rasad
Date Deposited: 12 Sep 2014 10:41
Last Modified: 12 Sep 2014 10:41
URI: http://irep.iium.edu.my/id/eprint/38238

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