Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms

Ahmad Rusmili, Muhamad Rusdi and Ting, Tee Yee and Mustafa, Mohd Rais and Hodgson, Wayne C. and Othman, Iekhsan (2014) Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms. Journal of Proteomics, 110. pp. 129-144. ISSN 1874-3919

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Abstract

Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms were found to contain a high percentage of three finger toxins, phospholipase A2 enzymes and Kunitz-type inhibitors. Smaller number of high molecular weight enzymes such as L-amino acid oxidase, hyaluronidases, and acetylcholinesterase were also detected in the venoms. We also detected some unique proteins that were not known to be present in these venoms. The presence of a natriuretic peptide, vespryn, and serine protease families was detected in B. candidus venom. We also detected the presence of subunit A and B of β-bungarotoxin and α-bungarotoxin which had not been previously found in B. fasciatus venom. Understanding the proteome composition of Malaysian krait species will provide useful information on unique toxins and proteins which are present in the venoms. This knowledge will assist in the management of krait envenoming. In addition, these proteins may have potential use as research tools or as drug-design templates.

Item Type:	Article (Journal)
Additional Information:	5591/38142
Uncontrolled Keywords:	Krait venom; Malaysian; Bungarus candidus; Bungarus fasciatus; Proteomics
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	11 Sep 2014 15:24
Last Modified:	20 Sep 2017 16:08
URI:	http://irep.iium.edu.my/id/eprint/38142

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