Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask

Mohamed, Vasila Packeer and Hashim, Yumi Zuhanis Has-Yun and Mohmad Saberi, Salfarin Ezrina and Ibrahim, Siti Nuramalis and Amid, Azura and Mel, Maizirwan (2014) Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask. Advances in Environmental Biology, 8 (3 ( Special Issue )). pp. 831-837. ISSN 1995-0756 E-ISSN 1998-1066

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Abstract

An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, it is hypothesized that glutamine and serum may affect CHO-K1 cell growth through Insulin-like Growth Factor-I (IGF-I)pathway. The effects of glutamine and serum on IGF-dependent CHO-K1 cell growth in T-flask were studied based on three-level Full Factorial Design generated by SIMCAP+v12 (Umetrics, Sweden). The combination factor of 0.50 mM glutamine and 10 % (v/v) serum was found to be the optimal condition producing 8.87 x 105 cells/ml. The CHO-K1 culture was also found to be able to grow in zero glutamine reaching 16.60 x 105 cells/ ml. Meanwhile, improvement of cell growth can also be achieved in reduced serum level with the presence of IGF-I protein.

Item Type:	Article (Journal)
Additional Information:	3774/38024
Uncontrolled Keywords:	CHO-K1 cells, cell growth, Insulin-like growth factor-I (IGF-I), glutamine, serum
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	03 Sep 2014 15:38
Last Modified:	19 Sep 2017 14:41
URI:	http://irep.iium.edu.my/id/eprint/38024

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