Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry

Malih, Ibitissam and Ahmad Rusmili, Muhamad Rusdi and Ting, Yee Tee and Saile, Rachid and Ghalim, Noreddine and Othman, Iekhsan (2014) Proteomic analysis of moroccan cobra naja haje legionis venom using tandem mass spectrometry. Journal of Proteomics, 96. pp. 240-252. ISSN 1874-3919

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Abstract

The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and database search. The sequence analysis of venom fractions revealed a highly complex venom proteome which counts a total of 76 proteins identified from database that can be assigned into 9 proteins families. We report the identification of: cobra venom factor (CVF), l-amino-acid oxidases (LAAO), acetylcholinesterase (AChE), snake venom metalloproteinases (SVMP), cysteine rich secretory proteins (CRISP), venom nerve growth factor (vNGF), phospholipases A2 (PLA2), vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cardiotoxins and cytotoxins. Comparison of these proteins showed high sequence homology with proteins from other African and Asian cobras. Further works are needed to assess the contribution of individual toxins in venom toxicity.

Item Type:	Article (Journal)
Additional Information:	5591/37199
Uncontrolled Keywords:	Venom proteome Naja haje legionis Moroccan cobra Tandem mass spectrometry
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User:	Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited:	08 Jul 2014 14:43
Last Modified:	20 Jun 2018 15:40
URI:	http://irep.iium.edu.my/id/eprint/37199

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