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Antioxidant and antimicrobial activities of shorea kunstleri

Daud, Siti Suria and Bakhtiar, M. Taher and Darnis, Deny Susanti and Mohamed Zaffar Ali, Mohamed Amiroudine and Tengku Zakaria, Tengku Muhamad Faris Syafiq (2014) Antioxidant and antimicrobial activities of shorea kunstleri. Journal of Coastal Life Medicine, 2 (8). pp. 638-644. ISSN 2309-5288 E-ISSN 2309-6152

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Abstract

Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM) and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate (FTC) assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration (MFC). Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity result in phenolic content of 8.34±0.003 g GAE/100 g of extract and 95.9±1.07% DPPH inhibition (IC50 value of 18.6 µg/ml), respectively. FTC assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of 74.2±0.35%, 74.0±0.10%, and 72.8±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against S. aureus, C. albicans, and C. tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against C. albicans and S. aureus (0.04 and 0.08 mg/ml, respectively) while DCM extract exhibited the highest activity towards C. tropicalis (MIC value of 0.63 mg/ml). Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/ml. Conclusion: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant and antimicrobial properties.

Item Type: Article (Journal)
Additional Information: 5209/37028
Uncontrolled Keywords: Shorea kunstleri, Phytochemicals, Antioxidant, Antimicrobial
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Kulliyyah of Science > Department of Biomedical Science
Kulliyyah of Science > Department of Chemistry
Depositing User: Dr Muhammad Taher
Date Deposited: 24 Jun 2014 21:17
Last Modified: 20 Jun 2017 14:37
URI: http://irep.iium.edu.my/id/eprint/37028

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