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Optimization of newcastle disease virus production in t-flask

Arifin, Mohd Azmir and Salim, Siti Hajar and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Syed Hassan, Sharifah (2011) Optimization of newcastle disease virus production in t-flask. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, Kuala Lumpur.

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Abstract

In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 4085/3643 (Proceedings of the 2nd International Conference on Biotechnology Engineering, ISBN: 9789834297831)
Uncontrolled Keywords: Newcastle disease virus; Asplin F strain; DF-1 cell; T-flask
Subjects: Q Science > QR Microbiology > QR355 Virology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Maizirwan Mel
Date Deposited: 11 Oct 2011 14:22
Last Modified: 19 Jan 2012 08:25
URI: http://irep.iium.edu.my/id/eprint/3643

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