α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells

Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin and Zakaria, Zainul Amiruddin (2014) α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells. In: 5th FIP Pharmaceutical Sciences World Congress (PSWC 2014), 13th-16th Apr. 2014, Melbourne, Australia.

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Abstract

α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibitory effect of the compound on lipid accumulation of 3T3-L1 preadipocytes was determined by Oil Red O staining method. Glucose uptake and free fatty acid release from the medium were determined by using 2-deoxy-D-[3H] glucose and adipolysis kit, respectively. Finally, the evaluation of related gene expression was carried out by the quantitative reverse transcriptase polymerase chain reaction (gRT-PCR). Depending on the dose, cell treated with α-mangostin was found to inhibit the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocyte. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake (p<0.05). In addition, α-mangostin increased the amount of free fatty acid released. qRT-PCR analysis showed that the α-mangostin reduced lipid accumulation by inhibition of PPARγ gene expression. The induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. In summary, these evidences proposed that α-mangostin might be beneficial for obesity management.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	5209/36403
Uncontrolled Keywords:	a-mangostin
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Pharmacy
Depositing User:	Dr Muhammad Taher
Date Deposited:	22 Apr 2014 09:57
Last Modified:	08 Jun 2022 14:24
URI:	http://irep.iium.edu.my/id/eprint/36403

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