Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi

Pauzi, Muhd Ridzuan and Hamzah, Hairul Aini and Shah, Anis and Mohd Hassan, Norazian and ., Roesnita and K. S., Aminah (2013) Antibacterial and antifungal properties of persicaria odorata leaf against pathogenic bacteria and fungi. The Open Conference Proceedings Journal, 4 (supp-2). pp. 71-74. ISSN 2210-2892

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Abstract

Persicaria odorata is a common plant and well known locally as “daun kesum” that is usually used in culinary and have various medicinal properties. This study is conducted to investigate the antimicrobial activity and the chemical constituent of the most active extract. The leaves were extracted using n-hexane, dichloromethane, methanol and water to produce the respective extracts. All extracts were tested against eight bacterial strains which include gram positive and gram negative bacteria and one fungal strain by using disc diffusion method. In this research erythromycin 15 µg, vancomycin 30 µg and gentamicin 10 µg were used as antibacterial standard whereas miconazole 50 µg were used as antifungal. The antimicrobial activity of the active extract is evaluated quantitatively by using broth microdilution assay. Gas Chromatography-Mass (GCMS) Spectroscopy analysis was used to analyze the chemical constituent of the most active extract. n-Hexane extract was found to be the most active extract which was active against S. aureus (29.3±0.57), S. epidermidis (32.6±1.52), S. pneumonia (11.3±1.52) and S. pyogenes (15.6±1.15). However, the all extracts are inactive against fungi. The extract produced minimum inhibitory concentration (MIC) of 100µg/ml against S. aureus and 50µg/ml each against S. epidermidis, S. pneumonia and S. pyogenes. GCMS analysis has identified Decanal, Caryophyllene, Dodecanal the major constituents of n-hexane extract. The results obtained in this study showed that P. odorata leaves have high potential to be used as natural antibacterial agent against bacterial infections.

Item Type:	Article (Journal)
Additional Information:	5342/35015
Uncontrolled Keywords:	Antibacterial and Antifungal
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Pharmacy
Depositing User:	Dr Hairul Aini Hamzah
Date Deposited:	30 Jan 2014 15:49
Last Modified:	23 Jul 2014 15:37
URI:	http://irep.iium.edu.my/id/eprint/35015

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