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In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load

Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah, Nasuruddin (2013) In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load. In: IIUM Research, Invention and Innovation Exhibition 2013, 19 - 20 February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM.

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Abstract

Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 5342/34879
Subjects: Q Science > QR Microbiology > QR355 Virology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Dr Hairul Aini Hamzah
Date Deposited: 28 Jan 2014 04:48
Last Modified: 28 Jan 2014 04:48
URI: http://irep.iium.edu.my/id/eprint/34879

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