Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells

Nakamichi, Naoto and Morii, Eiichi and Ikeda, Jun-Ichiro and Qiu, Ying and Mamat, Suhana and Tian, Tian and Fukuhara, Shirou and Aozasa, Katsuyuki (2009) Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells. Laboratory Investigation, 89 (3). pp. 327-336. ISSN 1530-0307 (O), 0023-6837 (P)

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Abstract

ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of plasma cells mainly located in the interfollicular space of lymphoid tissues. The number of ABCG2-high plasma cells increased in interleukin-6- (IL-6) rich lesions, such as Castleman's disease of plasma cell type. Plasma cells are subjected to endoplasmic reticulum (ER) stress when excess proteins are synthesized, and IL-6 stimulates protein synthesis. Therefore, the effect of IL-6 and ER stress on ABCG2 expression in plasma cells was examined. The expression level of ABCG2 increased by treatment with either IL-6 or ER stress inducers, and further increased with both. The promoter analysis revealed that the effect of IL-6 and ER stress inducers was mediated through the site overlapping XBP-1 and HIF-1 binding sequences. Knocked-down of ABCG2 by siRNA or ABCG2 inhibitor reduced plasma cell viability under ER stress. These suggest that ABCG2 is a surviving factor for plasma cells. To our knowledge, this is the first study reporting the effect of ER stress on ABCG2 expression.

Item Type:	Article (Journal)
Additional Information:	4912/34105
Uncontrolled Keywords:	ABCG2, ER stress, immunohistochemistry, lymph nodes, plasma cells
Subjects:	R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Basic Health Sciences (Until June 2011)
Depositing User:	Dr Suhana Mamat
Date Deposited:	13 Jan 2014 14:24
Last Modified:	13 Jan 2014 14:24
URI:	http://irep.iium.edu.my/id/eprint/34105

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