High performance liquid chromatographic method for Iso-a-Acids

Khatib, Alfi and Kim, Hye Kyong and Wilson, Erica G. and Verpoorte, Robert (2006) High performance liquid chromatographic method for Iso-a-Acids. Journal of Liquid Chromatography & Related Technologies, 29 (2). pp. 293-302. ISSN 1082-6076 print/1520-572X online

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Abstract

Using pure trans/cis-isocohumulone, trans/cis-isohumulone, and trans/cisisoadhumulone an isocratic HPLC system has been developed for quantitation of these compounds in hop extracts and beers. The mobile phase contains acetonitrile-water- H3PO4 (50 : 50 : 0.01, v/v/v) and is used with a Phenomenex Hypersil 5 m C18 column 250 � 4.6 mm, flow rate 1.5mL/min. Baseline separation of all 6 isomers was achieved with a total run time of 25 min. The UV spectrum of these pure compounds using this system are quite different. The effect of different mobile phase compositions on separation was investigated as well as the chromatographic parameters, detection limit, and linearity.

Item Type:	Article (Journal)
Additional Information:	6915/33743
Uncontrolled Keywords:	LC-UV, Iso-a-acids, Bitter acids, Hop, Humulus lupulus, Beer
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	06 Jan 2014 12:19
Last Modified:	06 Jan 2014 12:19
URI:	http://irep.iium.edu.my/id/eprint/33743

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