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Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity

Khatib, Alfi and Hoek, Arie C. and Jinap, Selamat and Sarker, Md. Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert (2010) Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic activity. Journal of Liquid Chromatography & Related Technologies, 33 (2). pp. 214-224. ISSN 1082-607

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Abstract

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl acetate=chloroform=methanol (28=28=44) as the second mobile phase.

Item Type: Article (Journal)
Additional Information: 6915/33317
Uncontrolled Keywords: asthma, centrifugal partition chromatography, fingerprinting, thin layer chromatography, tracheospasmolytic assay, Vitex trifolia Linn
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr. Alfi Khatib
Date Deposited: 09 Dec 2013 16:25
Last Modified: 09 Dec 2013 16:25
URI: http://irep.iium.edu.my/id/eprint/33317

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