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Increased of Bcl-2/Bax ratio corelated with decreased of lymphocyte apoptosis: a study in the bronchiolus and lung of asthmatic mice

Hasan, Taufiq Hidayat and Yuliarto, Saptadi and Barlianto, Wisnu and Sujuti, Hidayat and Kusuma, HMS Chandra (2013) Increased of Bcl-2/Bax ratio corelated with decreased of lymphocyte apoptosis: a study in the bronchiolus and lung of asthmatic mice. In: IIUM Research, Invention and Innovation Exhibition 2013, 19 - 20 February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM.

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The failure of lymphocyte apoptosis is one of the factors causing chronic airway inflammation in asthma. Some studies indicate the role of Bcl-2 in inhibition of lymphocyte apoptosis, but still little research is on the role of Bax and its relationship to Bcl-2 in asthma. The purpose of this study was to prove the role of Bcl-2-lymphocyte in inhibition of lymphocyte apoptosis and decrease in Baxlymphocytes in bronchiolus and lung of asthmatic mice. This study was a randomized control group design. Subjects were Balb/c mice which divided into 2 groups: non-asthma and asthma. Asthma group were sensitized with ovalbumin intraperitoneally on day 0 and 14, followed by inhalation every 2 to 3 days for 6 weeks. At week 8, all subjects terminated. Bcl-2 and Bax-lymphocytes expression were examined with immunohistochemical method, whereas apoptotic lymphocytes by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Statistical analysis used was the independent sample t-test and regression analysis, with 95% confidence interval. Bronchial and lung specimens were obtained from 18 subjects (9 from each group). The ratio of apoptotic lymphocytes decreased in the asthma group (p = 0.003), Bcl-2-lymphocytes increased in the asthma group (p < 0.001), and Bax-lymphocytes decreased in the asthma group (p = 0.003). There was a strong negative correlation (r = -0.66, r2 = 0.43, p = 0.003) between the Bcl-2 and lymphocyte apoptosis. There was also a strong negative correlation (r = -0.56, r2 = 0.35, p = 0.009) between the ratio Bcl-2/Bax and lymphocyte apoptosis. However, no significant relationship was found between Bax-lymphocytes and lymphocyte apoptosis (r = 0.36, r2 = 0.13, p = 0.15). Increasing the ratio of Bcl-2/Bax inhibit lymphocyte apoptosis where Bcl-2 plays more role than Bax.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 6714/33117
Uncontrolled Keywords: asthma, ovalbumin, lymphocyte apoptosis, Bcl-2, Bax
Subjects: Q Science > QR Microbiology > QR180 Immunology
R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine > Department of Paediatrics
Date Deposited: 24 Dec 2013 15:22
Last Modified: 24 Dec 2013 15:22
URI: http://irep.iium.edu.my/id/eprint/33117

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