Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina

Ahmad, Ahmad Shamsuddin and Musa, Najiah and Assaw, Suvik and Mohtar, Noor Azariyah and Yunus, Kamaruzzaman and Abd. Wahid, Mohd. Effendy and John, Akbar (2013) Antibacterial properties of selected mangrove plants against vibrio species and its cytotoxicity against artemia salina. World Applied Sciences Journal , 25 (2). pp. 333-340. ISSN 1818-4952

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Abstract

Emerging antibiotic resistance among the pathogenic microorganism has triggered the discovery of various antimicrobial compounds from marine source. Secondary metabolites from plants especially mangroves is traditionally being used widely as antimicrobials. Hence, present study was aimed to determine the antibacterial and cytotoxic properties of the selected mangrove plant leaves (Bruguiera cyclindrica, Sonneratia caseolaris, Luminitzera racemosa, Rhizophora apiculata, Avicennia alba, Acrostichum aureum, Nypa fruticans, Pandanus odoratissimus, Hibiscus tiliaceous and Derris trifoliate) against aquaculture pathogenic strains of genus. Methanol and aqueous extracts of leaves samples were prepared. The antibacterial activity was determined by using disc diffusion method against six pathogenic Gram-negative Vibrio species, such as Vibrio alginolyticus and V. parahaemolyticus (isolated from Scylla serrata, mangrove crab), V. alginolyticus and V. parahaemolyticus (from Giant prawn), V. alginolyticus (from Tiger prawn) and also V. parahaemolyticus (from Reference strain ATCC 17802). Methanolic extracts of S. caseolaris produced significant inhibition zone against all the pathogenic Vibrio spp. except for V. parahaemolyticus (from Giant prawn). Minimum inhibitory concentration test (MIC) and cytotoxicity (LC50) test using Artemia salina as a test animal in sterile ELISA (Enzyme-Linked Immunosorbent Assay) microplates showed the significant antimicrobial properties of methanolic extract of S. caesolaris. Overall, present study proved the active antimicrobial compounds in methanolic extract of S. caesolaris which could be used against Vibrio pathogens in aquaculture.

Item Type:	Article (Journal)
Additional Information:	5410/32668
Uncontrolled Keywords:	Antibacterial properties Vibrio sp. Solvent extract Mangrove plant Cytotoxicity Artemia salina
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Professor Dr. Kamaruzzaman Yunus
Date Deposited:	12 Nov 2013 12:42
Last Modified:	12 Nov 2013 12:42
URI:	http://irep.iium.edu.my/id/eprint/32668

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