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Lignin modifying enzyme activities by some Malaysian white rot fungi

Ruqayyah, Tijani I.D. and Jamal, Parveen and Alam, Md. Zahangir and Mirghani, Mohamed Elwathig Saeed (2011) Lignin modifying enzyme activities by some Malaysian white rot fungi. ACT-Biotechnology Research Communications, 1 (1). pp. 14-19. ISSN 1858-5965

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The possible biotechnological application of ligninolytic enzyme in a range of different fields has stimulated isolation and screening of potential microorganisms from local sources. Twelve white rot fungi (WRF) were isolated from around Gombak area (Malaysia) and sub cultured few times in order to obtain pure cultures. These strains were first screened on solid media plates for their lignin modifying enzymes by using Poly R-478 and ABTS (2, 2’azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid). Of the twelve white rot fungi tested seven were able to decolourize Poly R-478 and eleven showed significant ABTS-oxidation activities. The positive strains were then tested on liquid media for lignin peroxidases (LiP), manganese peroxidases (MnP) and laccase (Lac) activity. Six strains showed potential to secrete all the three enzymes. One strain was able to secrete only two enzymes and five strains secreted only one of the three enzymes.

Item Type: Article (Journal)
Additional Information: 2937/3251
Uncontrolled Keywords: White rot fungi, Lignin peroxidases, Manganese peroxidases, Laccase, Enzyme
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Prof.Dr. Parveen Jamal
Date Deposited: 20 Sep 2011 12:41
Last Modified: 20 Sep 2011 12:41
URI: http://irep.iium.edu.my/id/eprint/3251

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