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Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma

Chakrabarty, Uday Sankar and Das, Ayan and Bhaumik, Uttam and Chatterjee, Bappaditya and Nandi, Utpal and Pal, Tapan Kumar (2009) Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma. Chromatographia, 69 (9-10). pp. 853-858. ISSN 1612-1112 (O), 0009-5893 (P)

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A simple liquid chromatographic method for the determination of gemifloxacin (CAS number 175463-14-6) in human plasma has been developed. An aliquot quantity of 1 mL plasma sample was taken and 0.1 mL internal standard was added and mixed. 1 mL methanol was added to it. The mixture was then sonicated for 10 min followed by 20 min centrifugation at 5000 rpm (g = 3600). The supernatant layer was separated and filtered through simple filtration unit (membrane filter, 0.45 μm) and injected into the LC system consisting of Hypersil BDS, C18 (250 × 4.6 mm, 5 μm particle size) column, using 1% formic acid : methanol = 65:35 (v/v) as mobile phase with ultra violet detection at 328 nm. Lower limit of detection was 20 ng mL−1 and lower limit of quantitation was 50 ng mL−1. Maximum between-run precision was 14.614%. Mean extraction recovery was found to be 87.32 to 89.32%. Stability study showed that after three freeze-thaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 12 h and at −20 °C for 3 months. Before injecting into LC system, the processed samples were stable for at least 8 h. The method was used to perform bioequivalence study in human volunteers.

Item Type: Article (Journal)
Additional Information: 6923/31911
Uncontrolled Keywords: Column liquid chromatography Pharmacokinetics Plasma analysis Gemifloxacin
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Bappaditya Chatterjee
Date Deposited: 20 Sep 2013 12:15
Last Modified: 20 Sep 2013 12:15
URI: http://irep.iium.edu.my/id/eprint/31911

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