IIUM Repository

Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres

Elsaid Ali, Aimen Abdo and Muhammad, Taher and Helaluddin , Abul Bashar Mohammed and Mohamed, Farahidah (2012) Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres. Journal of Analytical & Bioanalytical Techniques, 3 (7). pp. 1-5. ISSN 2155-9872

[img] PDF (Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres) - Published Version
Restricted to Repository staff only

Download (1MB) | Request a copy

Abstract

A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitrile (A) and 0.1% v/v orthophosphoric acid (B), with the following pre-determined timed-gradient program: 70% (A) isocratic for 6 min, 70-75% (A) in 1.2 min, 75-80% (A) in 0.4 min, 80% (A) isocratic for 2.4 min, 80-70% (A) in 0.4 min, finally 70% (A)isocratic for 5 min, with a flow rate of 1 mL/min, detected at 320 nm by a UV detector. Linearity was obtained over the range of 1-200 μg/mL with r2=0.9995. The precision was achieved based on repeatability and intermediate precision with RSD of 0.13-0.6% and 0.57-1.2%, respectively. Percent recovery of 100.55% to 103.82% with RSD 0.086 - 0.15 implied high accuracy of the method. Limit of detection and limit of quantitation were 0.038 and 0.121 μg/ml, respectively suggesting good sensitivity of the method. The method is envisaged to be effectively used for routine quality control assay for encapsulated alpha-mangostin in PLGA microspheres.

Item Type: Article (Journal)
Additional Information: 4341/30074
Uncontrolled Keywords: Microencapsulation; Xanthone; Garcinia malaccensis
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Assoc Prof Farahidah Mohamed
Date Deposited: 05 Aug 2013 09:38
Last Modified: 17 Oct 2013 12:00
URI: http://irep.iium.edu.my/id/eprint/30074

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year