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Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells

Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Darnis, Deny Susanti and Ichwan, Solachuddin J. A. and Kaderi, Mohd Arifin (2013) Insulin mimicking activities of Cycloartane Triterpenoid in 3T3-L1 cells. In: IIUM Research, Invention and Innovation Exhibition 2013 (IRIIE 2013), 19-20 Feb 2013, Gombak, Kuala Lumpur.

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Abstract

Type 2 diabetes is a metabolic disorder characterized by insulin resistance, insulin action and caused by multifactorial etiology, including environmental factors, particularly diet and genetic components. Recently, most research on diabetes has focus on adipocyte which is used as a model for testing of insulin sensitivity and novel antidiabetic drugs. In this study, we investigated the effects of cycloartane triterpenoid on the adipocyte differentiation and glucose uptake in 3T3-L1 cells together with its underlying gene expression. The cells were treated for triglyceride accumulation with different concentration of the compound using Oil Red O staining assay. After 8-days, morphological changes and increase lipid accumulation were observed in these cells (p<0.05). Indeed, the intracellular lipid accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. Analysis of insulin-induced 2-deoxy-D-[3H] glucose uptake activities shows that cycloartane triterpenoids significantly (p<0.01) improved the glucose uptake with increasing the concentration of the compounds as compared to the basal. Further evaluation with the quantitative real time polymerase chain reaction (qRT-PCR) shows that mature 3T3-L1 cells treated with cycloart-24-en-3β-ol enhance pparγ and glut4 gene expression. As a result, it demonstrated that cycloartane triterpenoids enhance pparγ and glut4 gene expression. Taken together, these results suggest that cycloart-24-en-3β-ol derived from Garcinia malaccencis could improve insulin sensitivity through the activation of pparγ as a ligand and glut4.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 5209/29514
Uncontrolled Keywords: Pharmacy Education, Continuous Assessments, Mid- Semester Exam, End- Semester Exam, Students' Acadamic Performance
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 24 Dec 2014 08:37
Last Modified: 18 Sep 2015 08:17
URI: http://irep.iium.edu.my/id/eprint/29514

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