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Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus

Ahmad Raus, Raha and Ali, Abdul Manaf and Tan, Wen Siang and Mohd. Salleh, Hamzah and Eshagi, Majid and Yusoff, Khatijah (2007) Production of a panel of monoclonal antibodies against the nucleocapsid protein of heat resistant Newcastle disease virus. In: International Conference on Biotechnology Engineering (ICBioE’07), 8 - 10 May 2007, Kuala Lumpur.

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Abstract

A panel of six monoclonal antibodies against the nucleocapsid protein (NP) of Newcastle disease virus (NDV) strain AF2240 was produced by immunization of Balb/c mice with purified NP. Isotyping test revealed three of the monoclonal (mAbs) were IgG1 and the others were IgG2a. All of the mAbs recognized linearized NP epitopes. The reactivity of these mAbs with local isolates revealed that three mAbs recognized conserved antigenic sites and the others bind to antigenic sites which undergone considerable changes.

Item Type: Conference or Workshop Item (Full Paper)
Additional Information: 3911/29278
Uncontrolled Keywords: Monoclonal antibody; nucleocapsid; Newcastle disease virus
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology > QR355 Virology
S Agriculture > SF Animal culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Raha Ahmad Raus
Date Deposited: 25 Apr 2013 09:43
Last Modified: 25 Apr 2013 09:43
URI: http://irep.iium.edu.my/id/eprint/29278

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