Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cellsin vitro

Hashim, Yumi Zuhanis Has-Yun and Rowland, Ian R. and McGlynn, Hugh and Servili, Maurizio and Selvaggini, Roberto and Taticchi, Agnese and Esposto, Sonia and Montedoro, GianFrancesco and Kaisalo, Leena and Wähälä, Kristiina and Gill, Chris I.R. (2007) Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cellsin vitro. International Journal of Cancer, 122 (3). pp. 495-500. ISSN 00207136

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Abstract

Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 lg/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45–55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade.

Item Type:	Article (Journal)
Additional Information:	3774/2907
Uncontrolled Keywords:	olive oil phenolics; colorectal cancer; invasion; adhesion; extracellular matrix
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	14 Oct 2011 11:27
Last Modified:	14 Oct 2011 11:27
URI:	http://irep.iium.edu.my/id/eprint/2907

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