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Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes

Khaliq R., Abdul and Kafafy, Raed and Salleh, Hamzah Mohd. and Faris, Waleed Fekry (2012) Enhancing the efficiency of polymerase chain reaction using graphene nanoflakes. Nanotechnology, 23 (45). pp. 1-8. ISSN 0957-4484

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Abstract

The effect of the recently developed graphene nanoflakes (GNFs) on the polymerase chain reaction (PCR) has been investigated in this paper. The rationale behind the use of GNFs is their unique physical and thermal properties. Experiments show that GNFs can enhance the thermal conductivity of base fluids and results also revealed that GNFs are a potential enhancer of PCR efficiency; moreover, the PCR enhancements are strongly dependent on GNF concentration. It was found that GNFs yield DNA product equivalent to positive control with up to 65% reduction in the PCR cycles. It was also observed that the PCR yield is dependent on the GNF size, wherein the surface area increases and augments thermal conductivity. Computational fluid dynamics (CFD) simulations were performed to analyze the heat transfer through the PCR tube model in the presence and absence of GNFs. The results suggest that the superior thermal conductivity effect of GNFs may be the main cause of the PCR enhancement.

Item Type: Article (Journal)
Additional Information: 5259/28976
Uncontrolled Keywords: Computational fluid dynamics simulations; DNA products; Nanoflakes; PCR efficiency; Surface area; Tube model
Subjects: T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Depositing User: Dr Raed Kafafy
Date Deposited: 16 Aug 2013 15:02
Last Modified: 16 Aug 2013 15:02
URI: http://irep.iium.edu.my/id/eprint/28976

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