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Neuroregenerative property of haruan (Channa striatus spp.) traditional extract

Mohd Shafri, Mohd. Affendi and Mat Jais, Abdul Manan and Kyu, Kim Min (2011) Neuroregenerative property of haruan (Channa striatus spp.) traditional extract. Jurnal Intelek, 6 (1). pp. 77-83. ISSN 2231-7716

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Among key neurorestorative strategy in treating neurodegenerative diseases and brain trauma is neurostimulation using non-endogenous substances, thus improving regeneration and repair potential of neurons. The purpose of this work is to treat PC12 cells, an established neuronal differentiation model, with a traditional extract of haruan, Channa striatus spp, an indigenous riverine fish of Southeast Asian countries, and analyse PC12 cells’ morphologic and behavioural changes at different concentrations of the extract. PC12 cells were first cultured at 1x103 cells/cm2 density in 12 well plate in an incubator (5% CO2, 37°C), each containing 1ml of complete growth medium (Eagle’s Minimum Essential Medium with 10% foetal bovine serum), for 48 hours, to reach a 70% confluency. Media was removed and changed, and treated with 100μl, 200μl, 300μl and 400μl of haruan traditional extract (HTE). PC12 cells were observed under camera-equipped inverted microscope at 24, 48, 72 and 96 hours. Neurite outgrowth as well as growth behaviour were noted in comparison to negative (1ml complete growth medium) and positive (1ml cyclic AMP) controls. 100μl of HTE was demonstrated as the optimum concentration at which multiaxonal and multipolar cells and longest neurite outgrowth were seen. The result is suggesting that haruan traditional extract (HTE) is able to exert desirable effects in morphology and behaviour of PC12 cells and may potentially be useful therapeutically in the genesis and repair of neuron cells.

Item Type: Article (Journal)
Additional Information: 6845/28701
Uncontrolled Keywords: haruan, PC12, neurostimulation, neuroregeneration, regenerative medicine
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Mohd Affendi Mohd Shafri
Date Deposited: 23 Jan 2013 10:23
Last Modified: 13 Feb 2013 16:13
URI: http://irep.iium.edu.my/id/eprint/28701

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