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Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients

Zainuddin, Norafiza and Jaafar, Hasnan and Isa, Mohd. Nizam and Abdullah, Jafri Malin (2005) Malignant glioma: The involvement of loss of allelic heterozygosity and PTEN mutations in a group of Malay patients. Southeast Asian Journal of Tropical Medicine and Public Health, 36 (3). pp. 748-756. ISSN 0125-1562

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Abstract

Frequent loss of heterozygosity (LOH) and mutations of the tumor suppressor gene PTEN (phosphatase and tensin homologue deleted from chromosome 10) have been found in sporadic gliomas. The most documented regions of allelic losses include 9p21, 10q23-25 and 17p13 whereas PTEN aberrations are preferentially found in glioblastoma multiformes. This research aimed to detect the incidence of allelic losses on chromosomes 10q, 9p, 17p and 13q and mutations on exons 5, 6 and 8 of PTEN in malignant gliomas. Malignant glioma specimens obtained were classified histopathologically according to the WHO criteria. Each tumor was then subjected to polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis. Twelve of 23 (52%) malignant glioma cases showed allelic losses whereas 7 of 23 (30%) samples showed aberrant band patterns and mutations of PTEN. Four of these cases showed LOH in 10q23 and mutations of PTEN. The data on LOH indicated the involvement of different genes in the genesis of glioma whereas mutations of PTEN indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population.

Item Type: Article (Journal)
Additional Information: 4623/28467
Subjects: R Medicine > R Medicine (General)
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Norafiza Zainuddin
Date Deposited: 18 Jul 2013 12:37
Last Modified: 14 Sep 2013 13:37
URI: http://irep.iium.edu.my/id/eprint/28467

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