The effects of in vitro treatment of melastoma malabathricum on fibroblast proliferation

Abdull Rasad, Mohammad Syaiful Bahari and Kaderi, Mohd Arifin and Mat Alewi, Nur Aizura and Ahmad Roslen, Nurfariza and Abdullah, Samirah (2012) The effects of in vitro treatment of melastoma malabathricum on fibroblast proliferation. In: 2012 2nd International Conference on Environment Science and Biotechnology (ICESB 2012), 22 - 23 December 2012, Kuala Lumpur.

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Abstract

As the demands in wound care have increased, it is imperative to quickly address this issue at hand. Melastoma malabathricum has been claimed to have astounding treatment in wound repair. This study aims to determine its wound healing potentials by assessing and comparing the rate of proliferation of fibroblasts, before and after, treatment of M. malabathricum’s methanol and aqueous extracts. The study also aims to compare the potential proliferation of cells between different concentrations of extracts through Methylene Blue Assay (MBA). Results were analyzed through two-way ANOVA using SPSS. The results showed a marked proliferation of cells where 39.3 % and 20.7 % were seen at concentrations A3 and A4 respectively. Concentrations A3 and A4 showed a decrease to 13.2 % and 16.4 % at 48 hours to a negligible proliferation at 72 hours, which may indicate probable impairment in the wound treatment. The methanol extracts revealed a gradual dose-response inhibitory effect throughout all the concentrations, accounting for its toxicity effect on cells. This depicts a time and dose-dependent proliferative effect of M. malabathricum’s aqueous extract on fibroblasts, while its methanol extract had a toxic effect on the cells.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Additional Information:	4701/27906
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr. Mohammad Syaiful Bahari Abdull Rasad
Date Deposited:	27 Dec 2012 16:13
Last Modified:	15 Jul 2022 16:39
URI:	http://irep.iium.edu.my/id/eprint/27906

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