Jamal, Parveen and Saheed, Olorunnisola Kola and Alam, Md. Zahangir (2012) Banana peels: a new carbon source for bioprotein production by utilizing phanerochaete chrysosporium and candida utilis. In: 15th International Biotechnology symposium and Exhibition (IBS 2012), 16 September 2012 to 21 September 2012, Daegu, South Korea.
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Abstract
Bioprotein production from underutilized fruit peels is one of the most promising breakthroughs of biotechnological innovations. This research was carried out to increase the availability of high quality affordable proteins by fermentation of Banana peels (BP), which is a lignocellulosic waste generated by many food industries. Pretreatment of BP at different temperatures (20OC, 40OC, 60OC, 80OC and 100OC) was done to reduce the amount of saponin content. Forty percent reduction in saponin was observed at 1000C (from 9.5mg/g to 5.7mg/g). Solid state bioconversion was done by sequential mixed culture of Phanerochaete chrysosporium (PC) and Candida utilis (CU) and 88.93mg/g of bio-protein was produced. Further increment in bioprotein content from 88.93 to 111.78mg/g was achieved after screening of media compositions by using Placket Burman Design. Experimental results showed that BP, KH2PO4, FeSO4.7H2O, wheat flour and sucrose contributed at low level while urea, peptone, K2HPO4, KCl, NH4H2PO4, and MgS04.7H2O contributed at their high levels. Total soluble sugar (TOS), total reducing sugar (TRS) and total carbohydrate (TC) contents obtained were also high. This study showed a promising result with improved protein for selected potential substrate at optimum level of selected media parameters, which were 5% sucrose, 0.6% NH4H2PO4, 0.4% KCl and 0.5% MgS04.7H2O. Bioprotein from local agro-waste can be a good source of supplement and additive in the animal feed as well as additives in the chemical and pharmaceutical products.
Item Type: | Conference or Workshop Item (Full Paper) |
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Additional Information: | 2937/27840 |
Uncontrolled Keywords: | Banana peels, fermentation, bioprotein, media composition |
Subjects: | S Agriculture > S Agriculture (General) |
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Engineering > Department of Biotechnology Engineering |
Depositing User: | Prof.Dr. Parveen Jamal |
Date Deposited: | 02 Jan 2013 13:29 |
Last Modified: | 14 Feb 2013 07:49 |
URI: | http://irep.iium.edu.my/id/eprint/27840 |
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