Influence of multiple variables on characteristics of “Neurobionanoparticles” prepared by diffusion-solvent-evaporation technique

Mansor, Nur 'Izzati and Doolanea, Abd Monem and Wan Mohd Azam, Wan Imtiyaz and Abdul Latip, Siti Suhaila and Yusof, Nurul Amirah and Mohamed, Farahidah (2012) Influence of multiple variables on characteristics of “Neurobionanoparticles” prepared by diffusion-solvent-evaporation technique. In: International Conference and Exhibition on Pharmaceutical, Neutraceutical and Cosmeceutical Technology, 20 -21 Nov 2012, KLCC.

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Abstract

We had attempted to synthesize nanoparticle called “Neurobionanoparticle” (NBP), consisted of biodegradable polymers (PLGA and chitosan) and plant-origin material (Nigella Sativa), intended for delivery of plasmid DNA to the central nervous system (CNS). Several independent variables were employed namely, type of co-solvents (ethyl acetate, acetone and chloroform), concentration of N. sativa (0.3 %, 0.6%, 0.9% and 1.2 % v/v), concentration of chitosan (1% and 2% w/v) and homogenization speed of primary and secondary emulsion formation (8 000, 11 500 and 20 500 rpm). Size, zeta potential and surface morphology of the particles were characterized as a function of those multiple variables. The results suggested that fabrication of NBP using the diffusion-solvent-evaporation method employing ethyl acetate as co-solvent and speed 20 500 rpm successfully produced spherical nanoparticles with particle size of around 300 nm. At 2% w/v chitosan, high concentration of N. sativa (1.2% v/v) was found to moderately reduce the size of the nanoparticles to 200 nm. As a conclusion, appropriate set of variables could be employed to yield NBP of desirable characteristics.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	4341/27829
Uncontrolled Keywords:	Neurobionanoparticle, Nigella Sativa, PLGA, chitosan.
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Assoc Prof Farahidah Mohamed
Date Deposited:	21 Jan 2013 16:13
Last Modified:	13 Feb 2013 17:31
URI:	http://irep.iium.edu.my/id/eprint/27829

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